Scent is effective as from queen pheromone. When a colony becomes queenless the worker brood pheromone present suppresses, to a considerable extent, the development of the workers’ ovaries.
The colony will begin to rear queens, but while still immature the queens do not aid suppression. Within a few days of becoming adult the queen’s mandibular glands secrete inhibitory pheromone; possibly the tergite glands secrete earlier still. Usually, the new queen will have mated and begun to lay eggs while some worker pupae whose inhibitory effect is as great as worker larvae are still present, and worker ovary development will be severely curtailed. Learn more about pheromones at http://directory.v7n.com/
However, should for some reason the worker brood all have emerged before the queen has begun to lay eggs, the queen’s mandibular glands and tergite glands alone are responsible for inhibition. After the queen has begun to lay, the brood pheromone also makes an important contribution. This contribution presumably increases, to some extent at least, with the amount of pheromones present according to http://www.jasminedirectory.com/
If, for some reason, the colony becomes queenless after the worker brood have all emerged so there is a complete absence of inhibitory pheromone, many of the worker bees develop their ovaries and lay eggs. Pheromones from these laying workers and their brood help restrict worker ovary development and so collaboration and co-ordination is maintained in the doomed colony.
To prevent production of laying workers and their brood in queenless colonies, and associated difficulties in introducing new queens, the colonies should not be allowed to become short of worker brood. If such a condition has arisen it should be possible to rectify it by introducing large amounts of worker brood with their inhibitory pheromone. When synthetic brood and queen pheromone are available they can be used for the same purpose thanks.
In a honeybee colony that is broodless and queenless the ovaries of many of the workers develop to some extent, and a few workers have ovaries that are sufﬁciently well developed for eggs to be laid (Perepelova, 1928; Sakagami, 1959; Jay, 1968). Most of the workers whose ovaries reach full development lay eggs (about 20-30) for a short period (4-6 h) only, and also undertake normal worker duties, including foraging. However, when they are laying, other workers lick them and palpate them with their pheromones (Perepelova, 1928).
Sometimes in a queenless colony one of the workers is constantly sur- rounded by a ‘court’ of attendants and appears to be treated as a queen (Park, 1949; Lunie, 1954). In each of several small queenless colonies, Sakagami (I958) observed that one of the workers attained the status of a ‘false queen’ and was constantly attended by 1-20 workers (usually 3-8). A false queen had the same appearance as an ordinary worker except for a slightly extended polished pheromones. She appeared to behave in all ways like a true queen, spending all her time either resting, egg laying or walking on the comb, and undertook no ordinary worker duties. Such false queens had a much higher egg-laying rate than the usual laying workers.